Zinc Supplementation Effect on Salivary MMP-8 Level in Male Wistar Rats with Experimental Periodontitis
Nila Kasuma1, Tasha Octaricha2, Tofrizal3, Susi4, Aida Fitriana5, Fildzah Nurul Fajrin6, Eriyati Darwin7, Gian Ernesto8
1Nila Kasuma, Department of Oral Biology, Faculty of Dentistry, Andalas University, Indonesia
2Tasha Octaricha, Department of Oral Biology, Faculty of Dentistry, Andalas University, Indonesia
3Tofrizal, Department of Pathology Anatomy, Faculty of Medicine, Andalas University, Indonesia
4Susi,Department of Oral Biology, Faculty of Dentistry, Andalas University, Indonesia
5Aida Fitriana, Department of Oral Biology, Faculty of Dentistry, Andalas University, Indonesia
6Fildzah Nurul Fajrin, Department of Pathology Anatomy, Faculty of Medicine, Andalas University, Indonesia
7Eriyati Darwin, Department of Pathology Anatomy, Faculty of Medicine, Andalas University, Indonesia
8Gian Ernesto, Department of Oral Biology, Faculty of Dentistry, Andalas University, Indonesia
Manuscript received on 28 November 2020 | Revised Manuscript received on 06 December 2020 | Manuscript Accepted on 15 December 2020 | Manuscript published on 30 December 2020 | PP: 1-4 | Volume-1 Issue-1, December 2020 | Retrieval Number: A1011061120/2020©LSP
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© The Authors. Published by Lattice Science Publication (LSP). This is an open access article under the CC-BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
Abstract: Periodontitis is the 6th most prevalent disease in the world with 11,2% prevalence and 743 million individuals infected. MMP-8 is one of the inflammation biomarkers which is the most MMP found in periodontal diseases. Zinc act as MMP-8 inhibitors, which makes it an alternative treatment to inhibits MMP-8. This research aims to analyze the difference between MMP-8 level in healthy rat saliva and experimental periodontitis rat saliva, and also to analyze the difference between MMP-8 level in periodontitis rat saliva with zinc supplementation and in periodontitis rat saliva without zinc supplementation. This research was done with 30 male Wistar rats as a sample divided into 3 different groups i.e. control group, periodontitis without supplementation group, and periodontitis with supplementation group. Every group saliva specimen was collected and MMP-8 level was examined using ELISA method. MMP-8 level was statistically tested using one-way ANOVA. Statistic test showed a significant difference between the group with p<0,05. This research concludes that zinc supplementation is effective in suppressing MMP-8 level in periodontitis.
Keywords: MMP-8 level, Rat Saliva, Zinc Supplementation, Wistar Rats, Periodontitis